Impact of mydriasis in fluorescence lifetime imaging ophthalmoscopy
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime imaging ophthalmoscopy
Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores. It mainly derives its signal from lipofuscin at the level of the retinal pigment epithelium. Fundus autofluorescence, however, can...
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Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution lifetime images of thin tissue sections. This chapter concerns the in vivo time domain imaging of fluorescent contrast agents embedded in de...
متن کاملStroboscopic fluorescence lifetime imaging.
We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the relaxation rate of the fluorophores. The tech...
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FLUORESCENCE ophthalmoscopy is an important manoeuvre that can provide a great deal of information about certain pathological lesions of the retina and choroid, but simple observation of the fundus appearances after intravenous fluorescein injection has tended to be neglected in favour of photographic recordings. The apparatus required for the latter is expensive and time-consuming, and often r...
متن کاملFluorescence lifetime imaging in turbid media.
The lifetime of a f luorophore generally varies in different environments, making the molecule a sensitive indicator of tissue oxygenation, pH, and glucose. However, lifetime measurements are complicated when the f luorophore is embedded in an optically thick, highly scattering medium such as human tissue. We formulate the inverse problem for f luorescence lifetime tomography using diffuse phot...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2018
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0209194